Rewriting yeast central carbon metabolism for industrial ... - Nature
2016年9月21日 · Here we use four non-native metabolic reactions to rewire central carbon metabolism in S. cerevisiae, enabling biosynthesis of cytosolic acetyl coenzyme A (acetyl-CoA, the two-carbon isoprenoid...
2015年7月29日 · Horwitz et al. describe a streamlined method for using the CRISPR-Cas system in yeasts to integrate multiple DNA constructs into targeted locations in the genome with a single transformation. This method enabled rapid functional testing of combinations of mutations and prototyping of complete metabolic pathways.
Efficient Multiplexed Integration of Synergistic Alleles ... - Cell Press
2015年7月29日 · CRISPR-Cas genome engineering in yeast has relied on preparation of complex expression plasmids for multiplexed gene knockouts and point mutations.
High-yield chemical synthesis by reprogramming central …
2016年11月8日 · A recent study in Nature from Meadows et al. 5 at Amyris describes a groundbreaking effort to rewire carbon and energy metabolism in yeast in order to produce commercially relevant yields of...
Innovation trends in industrial biotechnology - Cell Press
2022年4月19日 · Even though Amyris did not profit financially from the project, it enabled the company to develop a yeast strain that could efficiently produce sesquiterpenes, a group of natural products to which artemisinic acid belongs. Amyris used this platform yeast strain for the production of other valuable sesquiterpenes (Figure 1A). In one case, they ...
Clean manufacturing powered by biology: how Amyris has …
2020年11月1日 · Founded in 2003 to create a reliable supply of cost-effective artemisinin for the treatment of malaria, Amyris has now scaled up and manufactured nine distinct fermentation molecules (terpenoids like farnesene, manool, bisabolol; terpenoid-based species like RebM; and non-terpenoid products) with multiple contract manufacturing organization (CMO...
Cell Systems - ScienceDirect
2015年7月29日 · CRISPR-Cas genome engineering in yeast has relied on preparation of complex expression plasmids for multiplexed gene knockouts and point mutations.
Amyris and the Yeast Alternatives : r/Amyris - Reddit
2023年1月3日 · KP has several advantages over SC (the only used yeast at Amyris until now), including better thermo- and osmose-tolerance, respiratory growth to extremely high cell densities, effective protein secretion, and the ability to express recombinant proteins at high levels from strong constitutive and inducible promoters.
Rewriting yeast central carbon metabolism for industrial ... - PubMed
2016年9月29日 · Here we use four non-native metabolic reactions to rewire central carbon metabolism in S. cerevisiae, enabling biosynthesis of cytosolic acetyl coenzyme A (acetyl-CoA, the two-carbon isoprenoid precursor) with a reduced ATP requirement, reduced loss of carbon to CO 2 -emitting reactions, and improved pathway redox balance.
Rewiring carbon metabolism in yeast for high level production of ...
2019年10月31日 · Here, the authors engineer baker’s yeast for high levels p-coumaric acid production by rewiring the central carbon metabolism and channeling more flux to the AAA biosynthetic pathway.