A western blot assay to measure cyclin dependent kinase activity in ...
2013年9月17日 · We developed a new recombinant protein substrate and adapted the technique of western blotting to measure Cdk1 activity quantitatively in biochemical assays and cell extracts. In designing a Cdk1/cyclin B1 substrate, we looked for …
A western blot assay to measure cyclin dependent kinase activity …
2013年9月17日 · We developed a quantitative method to measure the activity of cyclin-dependent kinases (Cdks) by western blotting, without radioisotopes. We prepared a recombinant protein substrate based upon the natural Cdk1 substrate, PP1Cα. By combining this substrate in a western blot method using fluorochrome …
Myc stimulates cell cycle progression through the activation of Cdk1 …
2019年12月10日 · Through genetic analysis based on loss of function of Cdk1 and Cdk2 along with conditional Myc expression, we show here the pivotal role of Cdk1 on p27 phosphorylation and its potential...
Characterization of Cdk1 antibodies using Western blotting. Western …
Western blots with five different Cdk1 antibodies are presented to illustrate the reactivity of antibodies to SNAP-tag fusion proteins purified from HEK 293 cells. The upper row shows Western...
We developed a quantitative method to measure the activity of cyclin-dependent kinases (Cdks) by western blotting, without radioisotopes. We prepared a recombinant protein substrate based upon the natural Cdk1 substrate, PP1Ca.
A western blot assay to measure cyclin dependent kinase activity …
2013年8月15日 · We developed a quantitative method to measure the activity of cyclin-dependent kinases (Cdks) by western blotting, without radioisotopes. We prepared a recombinant protein substrate based upon the natural Cdk1 substrate, PP1Cα.
(PDF) Measurement of Cdk1/Cyclin B Kinase Activity by Specific ...
2015年6月1日 · By combining this substrate in a western blot method using fluorochrome based antibodies and phospho-imager analysis, we measured the Km of ATP binding to Cdk1 to be 3.5μM.
Identification and characterization of the CDK1-BMAL1-UHRF1 …
2023年4月21日 · Western blot assay revealed that the knockdown of CDK1 increased P-BMAL1 (Ser42) expression without affecting the total BMAL1 level. In contrast, the knockdown of STK38 failed to affect BMAL1 phosphorylation (Figure 2A). Immunoprecipitation assay revealed that BMAL1 bound to CDK1, but not STK38 (Figure 2B).
A western blot assay to measure cyclin dependent kinase activity in ...
2013年8月14日 · We developed a quantitative method to measure the activity of cyclin-dependent kinases (Cdks) by western blotting, without radioisotopes. We prepared a recombinant protein substrate based upon...
Measurement of Cdk1/Cyclin B Kinase Activity by Specific
Protein phosphatase-1Cα (PP1Cα) is phosphorylated on threonine 320 by Cdk1/cyclin B and can be detected by western blotting using phospho-Thr 320-specific antibodies. With this information, we synthesized an artificial substrate (GST-PP1C-S) that contained the Cdk1 canonical consensus sequence found within PP1Cα at threonine 320 in frame to ...