Necroptosis, necrosis and secondary necrosis converge on …
2009年12月11日 · Necroptosis, necrosis and secondary necrosis following apoptosis represent different modes of cell death that eventually result in similar cellular morphology including rounding of the cell,...
Apoptosis and necrosis: Detection, discrimination and phagocytosis ...
2008年3月1日 · A particular difficulty in defining necrosis is that in the absence of phagocytosis apoptotic cells become secondary necrotic cells with many morphological features of primary necrosis. In this review, we present a selection of techniques that can be used to identify necrosis and to discriminate it from apoptosis.
Secondary necrosis of apoptotic neutrophils induced by the …
2009年7月6日 · We demonstrate that the human cathelicidin LL-37 induced rapid secondary necrosis of apoptotic human PMN and identify an essential minimal region of LL-37 required for this activity. Using these LL-37-induced secondary necrotic PMN, we characterize the consequence for macrophage inflammatory responses.
The immune response to secondary necrotic cells - PMC
2017年8月31日 · When apoptotic cells are not cleared in an efficient and timely manner, they progress to secondary necrosis and lose their membrane integrity. This results in a leakage of immunostimulatory, danger associated molecular patterns (DAMPs), similar to accidental (or primary) necrosis.
Secondary necrosis: The natural outcome of the complete …
2010年11月19日 · Secondary necrosis is the typical outcome of apoptosis in unicellular eukaryotes but, importantly, it may also occur in multicellular animals and has been implicated in the genesis of important human pathologies.
Analysis of Apoptosis and Necroptosis by Fluorescence-Activated …
Fluorescence-activated cell sorting (FACS) is a laser-based, biophysical technology that allows simultaneous multiparametric analysis. For the analysis of dying cells, fluorescently labeled Annexin V (Annexin V FITC) and propidium iodide (PI) are the most commonly used reagents.
Gasdermin E Does Not Limit Apoptotic Cell Disassembly by …
In contrast to the latter studies, we present here that GSDME is not involved in regulating secondary necrosis in human T cells and monocytes, and also unlikely in epithelial cells. Furthermore, GSDME is evidently not a negative regulator of …
Quantitative Analysis of Apoptosis and Necrosis in Live Cells Using ...
2022年9月11日 · Necrosis is characterized by the loss of membrane integrity even before the apoptotic caspases are activated, and the release of intracellular soluble cytoplasmic FRET probe occurs. When a stable non-soluble fluorescent probe of nonoverlapping spectra with that of FRET probe is simultaneously transfected into cells, necrosis and apoptosis can ...
A quantitative real-time approach for discriminating apoptosis and necrosis
2017年1月23日 · Large number of cellular and biochemical assays have evolved to determine apoptosis or necrosis for qualitative and quantitative purposes. A closer analysis of the assays and their performance...
Pulling the apoptotic trigger for necrosis - Nature
2017年1月11日 · This work shows that DFNA5 is a substrate of caspase 3 and drives necrosis after apoptosis induction, revealing that secondary necrosis is a regulated process downstream of apoptotic cell...