Poxvirus entry and membrane fusion - ScienceDirect
2006年1月5日 · The study of poxvirus entry and membrane fusion has been invigorated by new biochemical and microscopic findings that lead to the following conclusions: (1) the surface of the mature virion (MV), whether isolated from an infected cell or by disruption of the membrane wrapper of an extracellular virion, is comprised of a single lipid membrane ...
Syncytium formation induced by viral infection ~ ViralZone - Expasy
Syncytia result from the fusion of an infected cell with neighboring cells, leading to the formation of multinucleated, enlarged cells. This process is triggered by the surface expression of viral fusion proteins that are fusogenic directly at the host cell membrane.
The Biology of Varicella-Zoster Virus Replication in the Skin
The replication of varicella-zoster virus (VZV) in skin is critical to its pathogenesis and spread. Primary infection causes chickenpox, which is characterised by centrally distributed skin blistering lesions that are rich in infectious virus. ...
The Cytoplasmic Domain of Varicella-Zoster Virus Glycoprotein H ...
2014年5月29日 · When syncytia formation is regulated appropriately as in pOka-infected tissue, the virus is able to effectively spread and penetrate the skin creating the typical cutaneous lesions (Figure 14B). While this model requires further study, the present study demonstrates that tight regulation of syncytia formation through complementary functions of ...
Outer nuclear membrane fusion of adjacent nuclei in varicella …
2017年12月1日 · Varicella-zoster virus (VZV) is one typical syncytia-inducing virus that causes chickenpox and shingles in humans. Here, we report, for the first time, an interesting observation of apparent fusion of the outer nuclear membranes from juxtaposed nuclei that comprise VZV syncytia both in ARPE-19 human epithelial cells in vitro and in human skin ...
Structure–function analysis of varicella-zoster virus ... - PNAS
2011年10月24日 · Varicella-zoster virus (VZV) causes chicken pox and shingles. VZV is an α-herpesvirus closely related to herpes simplex virus 2, enabling prediction of the VZV gH structure by homology modeling. We have defined specific roles for each gH domain in VZV replication and pathogenesis using structure-based site-directed mutagenesis of gH.
The Membrane Fusion Step of Vaccinia Virus Entry Is ... - PLOS
2011年12月15日 · We determined that inhibitors of tyrosine protein kinases, dynamin GTPase and actin dynamics had little effect on binding of virions to cells but impaired membrane fusion, whereas partial cholesterol depletion and inhibitors of endosomal acidification and membrane blebbing had a severe effect at the later stage of core entry.
The Glycoprotein B Cytoplasmic Domain Lysine Cluster Is Critical …
2017年1月1日 · VZV syncytium formation, which has been implicated in the painful condition of postherpetic neuralgia, is regulated by the cytoplasmic domains of gB (gBcyt) via an immunoreceptor tyrosine-based inhibition motif (ITIM) and gH (gHcyt).
Development of a rapid image-based high-content imaging …
2023年2月1日 · Syncytia formation, a key hallmark of pox infection of cell lines, was identified as a pure phenotypic no-label assay that can be implemented to determine the efficacy of ST-246 and Ab-40 against VACV. The syncytia assay results correlated well with neutralizing HCI assay based on detecting VACV proteins.
Mutations Near the N Terminus of Vaccinia Virus G9 Protein …
2020年5月4日 · Poxviruses also encode proteins that localize in cell membranes and negatively regulate superinfection and syncytium formation. The vaccinia virus (VACV) A56/K2 fusion regulatory complex associates with the G9/A16 EFC subcomplex, but functional support for the importance of this interaction was lacking.