Creation of artificial karyotypes in mice reveals robustness
2022年9月20日 · Karyotyping analysis showed that while > 97% cells sustained a normal karyotype, the rest of cells carried chromosomal fusions, in which more than half were non-homologous Rb fusions that may...
Karyotyping Mouse Cells - CSH Protocols
Several methods for disaggregation of cells are described by Cronmiller and Mintz (1978). Additional variations on the methods for karyotyping and banding embryonal carcinomal cells are found in McBurney and Rogers (1982) and Robertson et al. (1983). For guidance in trypsinization, see Trypsinization of Cells Grown in Monolayer (Darlington 2006).
A sustainable mouse karyotype created by programmed ... - Science
2022年8月25日 · Here, we report programmed chromosome ligation in mice that resulted in the creation of new karyotypes in the lab. Using haploid embryonic stem cells and gene editing, we fused the two largest mouse chromosomes, chromosomes 1 and 2, and two medium-size chromosomes, chromosomes 4 and 5.
Chromosome territory reorganization through artificial …
2023年1月24日 · Karyotype analysis of the homozygous mice showed two fused Chr15-17 (Fig. 4d; Supplementary Fig. S5g). Apparently, homozygous, heterozygous and WT mice showed no differences in appearance...
Protocol for preparation and staining of chromosomes isolated …
2024年3月15日 · Here, we present a protocol for chromosome preparation, conventional G-banding, locus-specific fluorescent in situ hybridization, and spectral karyotyping for both mouse and human samples. This protocol optimizes the preparation of chromosomes from mouse and human cells for subsequent conventional and molecular cytogenetic analysis.
Spectral karyotyping analysis of human and mouse chromosomes
Spectral karyotyping (SKY), and the related multiplex fluorescence in situ hybridization (M-FISH), are chromosome-specific multicolor FISH techniques that augment cytogenetic evaluations of malignant disease by providing additional information and improved characterization of aberrant chromosomes that contain DNA sequences not identifiable using...
A reliable technique for karyotyping mouse oocytes prepared by a ...
2023年12月12日 · Here, we developed a novel karyotyping technique for mouse oocytes by applying multicolour fluorescence in situ hybridisation (FISH) to chromosome slides prepared by a gradual fixation/air-drying method, which is best suited to avoid rupture of oocyte membrane and artificial loss of chromosomes.
Multicolor karyotype analyses of mouse embryonic stem cells
We demonstrate that M-FISH is a sensitive and accurate method for a comprehensive karyotype analysis of ES cells and may minimize time, costs, and disappointments due to inadequate ES cell sources.
Multicolor karyotype analyses of mouse embryonic stem cells
We demonstrate that M-FISH is a sensitive and accurate method for a comprehensive karyotype analysis of ES cells and may minimize time, costs, and disappointment due to inadequate ES cell sources.
Creation of artificial karyotypes in mice reveals robustness of …
Mouse androgenetic haploid embryonic stem cells (AG-haESCs, also termed sperm-like stem cells) that carry only sperm genome are feasible for CRISPR-Cas9-mediated genetic manipulations, leading to the production of semi-cloned mice (SC mice) with corresponding genetic traits in one step through intracytoplasmic injection into oocytes (ICAHCI). 7 ...