天津工业生物所等首次解析环烯醚萜合成酶结构及催化机理
环烯醚萜合成酶（Iridoid Synthase, IRIS）是催化环烯醚萜类化合物合成中关键的成环酶，其底物为线状单萜10-oxogeranial，其产物为环状的nepetalactol，nepetalactol是多种天然产物的前体物质。 目前环烯醚萜类化合物主要是从中药中直接提取而来，生物合成方面的研究较少，尤其对于关键的IRIS是如何进行催化反应的，一直未见相关研究报导，了解IRIS的催化机理，将为生物合成环烯醚萜类化合物提供可能。 中国科学院天津工业生物技术研究所的郭瑞庭研究员带领的结构生 …
[Molecular cloning and expression analysis of iridoid synthase …
Iridoid synthase( IS),the key enzyme in the natural biosynthesis of vegetal iridoids,catalyzes the irreversible cyclization of 10-oxogeranial to epi-iridodial. In this study,we screened the Rehmannia glutinosa transcriptome data by BLASTn with Catharanthus roseus CrIS cDNA,and found four c …
An alternative route to cyclic terpenes by reductive ... - Nature
2012年11月21日 · Here we report the discovery of iridoid synthase, a plant-derived enzyme that generates the iridoid ring scaffold, as evidenced by biochemical assays, gene silencing, co-expression analysis and...
Iridodial - Wikipedia
Iridodial is an iridoid. It is produced from 8-oxogeranial by the enzyme iridoid synthase (IS). [1] Iridodial is one of the substrates for the enzyme iridoid oxidase (IO) which produces 7-deoxyloganetic acid.
Identification and functional characterization of three iridoid ...
2022年2月3日 · Iridoid synthase (ISY, EC 1.3.1.99) was first discovered in Catharanthus roseus (Apocynaceae) and identified as a noncanonical monoterpene cyclase that catalyzes a reductive cyclization reaction critical for the biosynthesis of the seco-iridoid backbone by using its physiological substrate 8-oxogeranial (Geu-Flores et al. 2012).
7-Deoxyloganetic acid synthase catalyzes a key 3 step oxidation …
2014年5月1日 · Functional expression of this gene in Saccharomyces cerevisiae confirmed its function as an authentic 7DLS that catalyzes the 3 step oxidation of iridodial–nepetalactol to form 7-deoxyloganetic acid. The identification of CYP76A26 removes a key bottleneck for expression of iridoid and related MIA pathways in various biological backgrounds.
Inverted stereocontrol of iridoid synthase in snapdragon
2017年9月9日 · We hypothesize that an epi-iridoid synthase that reduces C7 of 8-oxogeranial with R preference is involved in the biosynthetic pathway. We searched for a homologue of iridoid synthase that performs the R -selective reduction of the iridoid precursor 8-oxogeranial.
Identification and Characterization of the Iridoid Synthase …
From these candidates, we have functionally characterized the olive homologue of iridoid synthase (OeISY), an unusual terpene cyclase that couples an NAD (P)H-dependent 1,4-reduction step with a subsequent cyclization, and we provide evidence that OeISY likely generates the monoterpene scaffold of oleuropein in olive fruits.
Iridoid Synthase Activity Is Common among the Plant …
2015年1月5日 · The monoterpenoid branch of the alkaloid pathway leads to the secoiridoid secologanin and involves the enzyme iridoid synthase (IS), a member of the progesterone 5β-reductase (P5βR) family. IS reduces 8-oxogeranial to iridodial. Through transcriptome mining, we show that IS belongs to a family of six C. roseus P5βR genes. Characterization of ...
Biocatalytic routes to stereo-divergent iridoids - Nature
2022年8月11日 · Here we use a combination of naturally occurring and engineered enzymes to produce seven of the eight possible nepetalactol or nepetalactone stereoisomers. These enzymes open the possibilities for...
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