DNA Methylation Analysis | Genome-wide methylation …
What is DNA methylation analysis? DNA methylation analysis allows scientists to gain valuable insight into gene regulation and identify potential biomarkers. Aberrant DNA methylation has been implicated in many disease processes, including cancer, obesity, and addiction.
A qPCR technology for direct quantification of methylation in untreated DNA
2023年8月24日 · We present a qPCR technology, EpiDirect®, that allows for direct PCR quantification of DNA methylations using untreated DNA. EpiDirect® is based on the ability of Intercalating Nucleic Acids...
Quantitative comparison of DNA methylation assays for …
2016年6月27日 · Here we systematically compared and evaluated the most promising assays for measuring DNA methylation in large cohorts, clinical diagnostics and biomarker development.
A Practical Guide to the Measurement and Analysis of DNA Methylation
In this practical guide to the measurement and analysis of DNA methylation, the goal is to convey basic concepts in DNA methylation biology and explore genome-scale bisulfite sequencing as the current gold standard for assessment of DNA methylation.
DNA methylation data by sequencing: experimental …
2019年12月12日 · Specific methods allow scientists to identify novel genome-wide epigenetic patterns of DNA methylation down to single-nucleotide resolution. DNA methylation is the most researched epigenetic mark involved in various processes in the human cell, including gene regulation and development of diseases, such as cancer.
Analysing and interpreting DNA methylation data - Nature
2012年9月18日 · Bioinformatic methods for visualization of DNA methylation data facilitate quality assessment and help to pinpoint global trends in the data. By combining stringent statistical methods with...
A Practical Guide to the Measurement and Analysis of DNA Methylation ...
2019年4月23日 · DNA methylation is a fundamental, dynamic epigenetic mark that is involved in myriad developmental, homeostatic, and pathologic processes. A detailed mechanistic understanding of the biology of DNA methylation as a biomarker or causal substrate requires methods to measure and analyze DNA methylation using low-bias and high-resolution techniques.
DNA methylation: a timeline of methods and applications
2011年10月24日 · The techniques for DNA methylation analysis have developed from the ability to simply measure the amount of 5-methylcytosine within a particular genome in the early 1980s to a variety of basic comparative methods involving methylation-sensitive restriction enzymes, immunoprecipitation or bisulfite sequencing usually in combination with PCR up ...
DNA Methylation Validation Methods: a Coherent Review …
2019年10月1日 · Here, we present a practical overview of four commonly used validation methods for DNA methylation assessment: methylation specific restriction endonucleases (MSRE) analysis, pyrosequencing, methylation specific high-resolution DNA melting (MS-HRM) and quantitative methylation specific polymerase chain reaction (qMSP).
RnBeads 2.0: comprehensive analysis of DNA methylation …
2019年3月14日 · High-throughput assays enable genome-scale DNA methylation analysis in large numbers of samples. Here, we describe a new version of our RnBeads software - an R/Bioconductor package that implements start-to-finish analysis workflows for Infinium microarrays and various types of bisulfite sequencing.