Cas9 - Wikipedia
Cas9 is a 160 kDa protein that cuts DNA and alters a cell's genome. It is part of the CRISPR system that defends bacteria against viruses and plasmids, and is widely used in genetic engineering applications.
Cas9 Protein: Structure, Function, Types and Importance
2022年2月9日 · Learn about the Cas9 protein, a nuclease that cleaves DNA using RNA guidance and PAM sequence. Discover the different types of Cas9 nucleases, their functions, mechanisms and applications in gene editing and therapy.
CRISPR/Cas9 therapeutics: progress and prospects
2023年1月16日 · Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases by...
What is CRISPR/Cas9? - PMC - PubMed Central (PMC)
CRISPR/Cas9 is a gene-editing technology which involves two essential components: a guide RNA to match a desired target gene, and Cas9 (CRISPR-associated protein 9)—an endonuclease which causes a double-stranded DNA break, allowing modifications to the genome (see figure 1).
A Review on the Mechanism and Applications of CRISPR/Cas9…
Cas9 is a protein associated with the CRISPR adaptive immune systems of S. pyogenes and is referred to as SpyCas9 protein. The Spy Cas9 protein had a large multifunctional domain of 1368 amino acids and acts as a DNA endonuclease in natural and artificial CRISPR/Cas systems.
R-loop formation and conformational activation mechanisms of Cas9
2022年8月24日 · Cryo-electron microscopy structures of Streptococcus pyogenes Cas9 in multiple DNA-bound states provide insights on the mechanism of Cas9 activation by target DNA.
CRISPR–Cas9: A History of Its Discovery and Ethical Considerations …
The most studied protein from the Cas group is the Cas9 nuclease; in the ~20 years since the discovery of the cas genes more than 20,000 articles in the PubMed system mention the name Cas9 in one context or another.
The chemistry of Cas9 and its CRISPR colleagues - Nature
2017年10月4日 · As a result of their programmable ability to cut specific DNA and RNA sequences, Cas9 and related single-subunit effector proteins from CRISPR–Cas systems have been widely adopted for...
A Review on the Mechanism and Applications of CRISPR/Cas9
2022年9月27日 · Learn about the mechanism and applications of various Cas proteins that mediate CRISPR/Cas system for genome editing. Compare the features, advantages, and limitations of Cas9, Cas12, Cas13, and Cas14 proteins in different fields, such as virus detection and gene therapy.
Cas9 - an overview | ScienceDirect Topics
Cas9 is a protein that can cleave DNA or RNA based on a guide RNA sequence. Learn about its applications in gene editing, transcriptional regulation, biosensing, and food analysis.