A fast and cost-effective method for apolipoprotein E ... - Nature
2018年4月13日 · Apolipoprotein E (apoE) is a 34 kDa glycoprotein involved in lipid metabolism. The human APOE gene encodes for three different apoE protein isoforms: E2, E3 and E4. The...
An optimized method for PCR-based genotyping to detect human APOE …
2023年11月1日 · Apolipoprotein E genotyping method by real time PCR, a fast and cost-effective alternative to the TaqMan and FRET assays
Apolipoprotein E genotyping method by real time PCR, a fast ... - PubMed
2009年10月15日 · The apolipoprotein E gene (APOE) polymorphism genotyping has an allegedly important predictive value for coronary heart disorders and Alzheimer's disease. We developed a simple, fast, cost-effective and suited for high-throughput protocol for determining APOE genotypes by Real Time PCR monitored by …
A rapid and cost-effective method for genotyping apolipoprotein E …
2016年1月12日 · We have developed an APOE genotyping method that is based on allele-specific PCR methodology adapted to Real Time PCR monitored by TaqMan probe. Rather than using TaqMan probes specific for the two polymorphic sites, only one TaqMan probe is used as the polymorphic alleles are recognized by site-specific PCR primers.
A rapid and cost-effective method for genotyping apolipoprotein E …
2016年1月12日 · Human APOE gene is polymorphic at two single nucleotides (rs429358 and rs7412) resulting in three different alleles (ε2, ε3 and ε4). ApoE isoforms modulate the risk for a variety of vascular and neurodegenerative diseases; thus, APOE genotyping is crucial for predicting disease risk and designing individualized therapy based on APOE genotype.
基于TaqMan-TAMRA探针技术的ApoE基因快速分型方法研究
目的:建立基于TaqMan-TAMRA探针技术的快速,准确的载脂蛋白E (ApoE)基因型检测方法, 为临床诊断,治疗和预防ApoE基因相关疾病提供依据.方法对1049例血液样本提取DNA后,利用TaqMan-TAMRA探针对ApoE基因进行检测 和分型,并利用测序法进行验证.结果1049例样本中,共检出1048例, ApoE基因的6种基因型 (ε3/ε3,ε2/ε3,ε3/ε4,ε2/ε4,ε2/ε2,ε4/ε4)所占比例分别为68.64%,13.54%, 14.97%,1.72%,0.76%,0.38%.TaqMan-TAMRA探针法分型结果与测序结果完 …
TaqMan systems for genotyping of disease-related …
We have developed TaqMan assay systems for the single nucleotide polymorphisms -219G/T, located in the promoter of the apolipoprotein E gene, 113G/C, present in the transcriptional enhancer element of intron 1, 334T/C, determining Cys or Arg as amino acid residue 112 of mature apolipoprotein E, and 472C/T, determining Arg or Cys as residue 158.
Apolipoprotein E genotyping method by Real Time PCR, a fast …
2009年10月15日 · The apolipoprotein E gene (APOE) polymorphism genotyping has an allegedly important predictive value for coronary heart disorders and Alzheimer's disease. We developed a simple, fast, cost-effective and suited for high-throughput protocol for determining APOE genotypes by Real Time PCR monitored by SYBR ® Green.
To allow for simple and accurate genotyping of apoE SNPs, we established PCR-based 1-tube assay systems involving allele-specific oligonucleotide probes la-beled with fluorogenic reporter dyes...
2023年6月13日 · the APOE genotypes play a crucial role in determining the genetic risk associated with various diseases. Hence, it is worth evaluating the allelic variation in different populations. The primary methods for assessing the genetic variation of APOE isoforms were based on protein isoelectrofocusing [15]. Protein