Growth Differentiation Factor 9 and Its ... - Oxford Academic
Feb 1, 2007 · As an oocyte-specific growth factor, GDF9 plays critical roles in controlling folliculogenesis in mammals. In the present study, we cloned a 2.1-kb cDNA of the zebrafish GDF9 homolog (Gdf9, gdf9), which shares ~60% homology with that of …
Loss of growth differentiation factor 9 causes an arrest of early ...
Dec 15, 2022 · Phenotype analysis of zebrafish gdf9 mutant (gdf9-/-) showed that similar to GDF9 in the mouse, Gdf9 also plays an essential role in controlling early folliculogenesis in fish. The disruption of gdf9 gene caused a complete arrest of follicle development at the PG stage without any signs of follicle activation to enter the fast-growing SG phase.
Expression analysis of growth differentiation factor 9 (Gdf9/
Feb 3, 2017 · To demonstrate if the oocyte-derived Gdf9 plays a role in regulating the expression of cyp19a1a and amh, we prepared recombinant zebrafish Gdf9 (rzfGdf9) with the CHO cells. The activity of rzfGdf9 was assessed by its stimulation of Smad2 phosphorylation in …
Genetic evidence for differential functions of figla and nobox in ...
Nov 21, 2023 · Both figla-/- and nobox-/- mutants showed all-male phenotype in zebrafish due to increased dominance of the male-promoting pathway. The early diversion towards males in these mutants has...
Expression analysis of growth differentiation factor 9 (Gdf9/gdf9 ...
Feb 1, 2017 · We have reported that Gdf9/gdf9 is an oocyte-specific factor in the zebrafish, similar to that of mammals. Whether and how Gdf9 is involved in gonadal differentiation is unknown. In this study, we compared the expression levels of gdf9, cyp19a1a, and amh among several other sex-related genes in the gonads before, during, and after sex ...
Establishment of transgenic zebrafish (Danio rerio) models expressing ...
Dec 1, 2021 · Gdf9, an oocyte-secreted factor, is a potent regulator of folliculogenesis in both mammals and fish. By contrast, Gsdf is expressed by the gonadal somatic cells (i.e., Sertoli cells in the testis and granulosa cells in the ovary) that support germ cell development.
Loss of growth differentiation factor 9 causes an arrest of early ...
Dec 15, 2022 · In this study, we created a mutant for Gdf9 gene (gdf9-/-) in zebrafish using TALEN approach. The loss of Gdf9 caused a complete arrest of follicle development at primary growth (PG) stage. These follicles eventually degenerated, and all mutant females gradually changed to males through sex reversal, which could be prevented by mutation of the ...
斑马鱼卵巢中的生长分化因子9及其时空表达和调控。,Biology of …
Nov 10, 2006 · As an oocyte-specific growth factor, GDF9 plays critical roles in controlling folliculogenesis in mammals. In the present study, we cloned a 2.1-kb cDNA of the zebrafish GDF9 homolog (Gdf9, gdf9), which shares approximately 60% homology with that of mammals in the mature region.
Loss of growth differentiation factor 9 causes an arrest of early ...
Jul 2, 2022 · In this study, we created a mutant for Gdf9 gene (gdf9-/-) in zebrafish using TALEN approach. The loss of Gdf9 caused a complete arrest of follicle development at primary growth (PG) stage.
Growth differentiation factor 9 and its spatiotemporal ... - PubMed
Growth differentiation factor 9 (GDF9) is a member of the transforming growth factor beta (TGFB) superfamily. As an oocyte-specific growth factor, GDF9 plays critical roles in controlling folliculogenesis in mammals. In the present study, we cloned a 2.1-kb cDNA of the zebrafish GDF9 homolog (Gdf9, …