Chromatin Immunoprecipitation Sequencing (ChIP-Seq) - Illumina
What is ChIP-Seq? By combining chromatin immunoprecipitation (ChIP) assays with sequencing, ChIP sequencing (ChIP-Seq) is a powerful method for identifying genome-wide DNA binding sites for transcription factors and other proteins. Following ChIP protocols, DNA-bound protein is immunoprecipitated using a specific antibody.
Whole Genome ChIP-Seq. Chromatin immunoprecipitation is a powerful method to selectively enrich for DNA sequences bound by a particular protein in living cells. However, the widespread use of this method has been limited by the lack of a suficiently robust method to identify all of the enriched DNA sequences. The ChIP process enriches specific ...
ChIP-Seq data is less complex than other types of massively parallel sequencing data since analysis consists of determining a census count of tags from a relatively purified DNA sample. There are, howev-er, some informatics steps that must be followed to extract meaningful data from the raw sequence reads. Figure 1: Chip-Seq Tags in Bed Format ...
Paired-End vs. Single-Read Sequencing Technology - Illumina
Single-read sequencing involves sequencing DNA from only one end, and is the simplest way to utilize Illumina sequencing. Unlike single-read seqeuncing, paired-end sequencing allows users to sequence both ends of a fragment and generate high-quality, alignable sequence data. Paired-end sequencing facilitates detection of genomic rearrangements ...
ChIRP-Seq - Illumina
ChIRP-Seq. ChIRP, also commonly referred to as ChIRP-seq, is a protocol to detect the locations on the genome where ncRNAs, such as lncRNAs, and their proteins are bound. In this method, samples are first crosslinked and sonicated. Biotinylated tiling oligos are hybridized to the RNAs of interest, and the complexes are captured with ...
RNA Sequencing | RNA-Seq methods & workflows - Illumina
RNA sequencing (RNA-Seq) is revolutionizing the study of the transcriptome. A highly sensitive and accurate tool for measuring expression across the transcriptome, it is providing scientists with visibility into previously undetected changes occurring in disease states, in response to therapeutics, under different environmental conditions, and across a wide range of other study …
ATAC-Seq for Chromatin Accessibility Analysis - Illumina
The assay for transposase-accessible chromatin with sequencing (ATAC-Seq) is a popular method for determining chromatin accessibility across the genome. By sequencing regions of open chromatin, ATAC-Seq can help you uncover how chromatin packaging and other factors affect gene expression. ATAC-Seq does not require prior knowledge of regulatory ...
ChIP-Seq/ChIP-exo/HT-ChIP - Illumina
ChIP-Seq is a well-established method to map specific protein-binding sites. It has given rise to a vast number of derivatives, such as AHT-ChIP-Seq 1, BisChIP-Seq 2, CAST-ChIP 3, ChIP-BMS 4, ChIP-BS-seq 5, ChIPmentation 6, Drop-ChIP 7, Mint-ChIP 8, PAT–ChIP 9, reChIP-seq 10, scChIP-seq 11, and X-ChIP 12.Sequential ChIP-seq (reChIP) can also show the association …
DNA Sequencing | Understanding the genetic code - Illumina
ChIP Sequencing. By combining chromatin immunoprecipitation (ChIP) assays and sequencing, ChIP-Seq is a powerful method to identify genome-wide DNA binding sites for transcription factors and other proteins.
Chromatin Immunoprecipitation Sequencing (ChIP-Seq)
Combining chromatin immunoprecipitation (ChIP) assays with sequencing, ChIP-Seq is a powerful method for genome-wide surveys of gene regulation.