Capturing the start point of the virus–cell interaction with
2022年11月10日 · Here we present an active-feedback single-particle tracking method with simultaneous volumetric imaging of the live cell environment called 3D-TrIm to address this knowledge gap. 3D-TrIm...
A 3D structural SARS-CoV-2–human interactome to explore ... - Nature
2021年11月29日 · We highlight the utility of computational and structural approaches to model the SARS-CoV-2–human interactome, from the interaction between the SARS-CoV-2 spike protein (S) and human angiotensin ...
Design of customized coronavirus receptors | Nature
2024年10月30日 · In this study, we built receptors with customized specificity by grafting virus-binding domains (VBDs) on various transmembrane scaffolds, and demonstrate that ACE2 can be entirely substituted...
Three-Dimensional Visualization of Viral Structure, Entry, and ...
Cryo-ET involves the flash-freezing of a biospecimen (a virus, cell, tissue, or protein), imaging the sample through a tilt-series using an electron microscope, and finally aligning and merging the images using computational techniques to reconstruct a 3D image.
3D engineered tissue models for studying human-specific …
2023年3月1日 · 3D in vitro infectious models recapitulate the complex cell-virus-immune interaction. Viral infections cause damage to various organ systems by inducing organ-specific symptoms or systemic multi-organ damage.
Capturing the start point of the virus-cell interaction with high …
This multi-modal approach provides unique value which cannot be extracted by its independent components alone, and as demonstrated here provides unprecedented glimpses into the initial moments of the virus-cell interaction. Using 3D-TrIm, we acquired over 3000 viral trajectories and demonstrate the benefits of how this contextualized tracking ...
Advances in high-throughput methods for the identification of virus ...
2019年12月21日 · Recent advances in targeted gene perturbation, high-throughput screening, and mass spectrometry have facilitated the discovery of virus receptors in recent years. In this review, we compare the current methods used within the field to identify virus receptors, focussing on genomic- and interactome-based approaches.
Re-Engineered Pseudoviruses for Precise and Robust 3D Mapping …
2023年11月1日 · The angiotensin-converting enzyme 2 (ACE2) was identified as entry receptor on cells enabling binding and infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via trimeric spike (S) proteins protruding from the viral surface.
Single-molecule 3D imaging of HIV cellular entry by liquid-phase ...
The accumulated densities observed within the concave surface of the cell may be due to cell receptor binding (hairpin-shaped density indicated by cyan arrows in Fig. 4a) with viral glycoprotein (white dots on virus surface) and the triggered downstream binding of proteins, such as the quatrefoil-shaped cell receptor (indicated by orange arrow ...
Virus–Receptor Interactions: The Key to Cellular Invasion
PtdSer receptors, including T-cell immunoglobulin and mucin domain (TIM) and TYRO3, AXL, and the MERTK family of receptor tyrosine kinases (TAMs), have both been shown to serve as receptors for enveloped viruses from multiple virus families and …