Structure and function of a neocortical synapse | Nature
2021年1月13日 · Here we combine slice electrophysiology of synaptically connected pyramidal neurons in the mouse somatosensory cortex with correlated light microscopy and high-resolution electron microscopy...
Deep learning-based synapse counting and synaptic ultrastructure ...
2023年1月15日 · Transmitted light microscopy and even super-resolution laser scanning microscopy offer insufficient resolution to reveal the ultrastructure of synapses; electron microscopy does have Ångstrom resolution and thus allows the observation of detailed synaptic ultrastructures and careful measurement of their morphology (Nellist et al., 1995a ...
Estimation of the number of synapses in the hippocampus and
2020年8月19日 · Synapses can be identified with several methods, including genetic labeling of synaptic scaffolding proteins and electron microscopy (EM). Membrane-associated guanylate kinases (MAGUKs) are a...
SyConn2: dense synaptic connectivity inference for volume electron ...
2022年10月24日 · SyConn2 is a machine learning-based framework for inferring and analyzing the connectomes contained in a volume electron microscopy dataset of brain tissue, for example from the zebra finch.
Multi-scale light microscopy/electron microscopy neuronal imaging …
2022年1月21日 · Electron microscopy (EM) provides an unparalleled resolution to trace nanometer-thin neuronal processes and identify a synapse unambiguously.
SynapsEM: Computer-Assisted Synapse Morphometry - Frontiers
2020年12月17日 · Morphometry is typically performed on an electron micrograph of a single synaptic profile from a 30–70 nm-thick section. Analyses from ~200 synaptic profiles are then summed, and results are compared between controls and experimental samples, such as mutants or drug-treated neurons.
Studying synapses in human brain with array tomography and electron …
2013年6月6日 · Parallel processing for transmission electron microscopy (TEM) using a protocol modified to preserve structure in human samples allows complimentary ultrastructural studies. Incorporation of array tomography and TEM into brain banking is a potent way of phenotyping synapses in well-characterized clinical cohorts to develop clinico-pathological ...
Synapse classification and localization in Electron Micrographs
2014年7月1日 · This paper explored the problem of synapse detection in Electron Micrographs by addressing the problem of attribute based synapse description and fast synapse localization. Firstly, a novel attribute based synaptic junction descriptor that models the visual characteristics of clefts, ribbons and vesicles was presented.
The structural features of a synapse help determine its function. Synapses are extremely small and tightly packed with vesicles and other organelles. Visualizing synaptic structure requires imaging by electron microscopy, and the features in micrographs must be quantified, a process called morphometry. Three parameters are typically assessed from
In this paper we propose novel methods for synapse detection and localization, an important problem in connectomics. We first propose an attribute based descriptor for characterizing synaptic junctions. These descriptors are task specific, low dimensional and can be scaled across large image sizes.
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