Phalloidin staining protocol - Abcam
2021年11月17日 · Phalloidin is a highly selective bicyclic peptide used for staining actin filaments (also known as F-actin). It binds to all variants of actin filaments in many different species of animals and plants.
Phalloidin Conjugates for Actin Staining - Thermo Fisher Scientific
Phalloidin Conjugates for Actin Staining. Cytoskeleton. There are a variety of fluorescent and biotinylated phalloidins that label F-actin. These conjugates are water-soluble, allowing for convenient labeling, identifying, and quantitating of F-actin in tissue sections, cell cultures, and cell-free experiments.
Phalloidin - Wikipedia
The properties of phalloidin make it a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy.
Actin Staining Protocol | Thermo Fisher Scientific - US
Fluorescent and biotinylated phallotoxins stain F-actin at nanomolar concentrations and are extremely water soluble, thus providing convenient probes for labeling, identifying, and quantitating F-actin in tissue sections, cell cultures, or cell-free experiments. 1-3 Labeled phallotoxins have similar affinity for both large and small filaments ...
Cell assay, fix and stain (DAPI, Phalloidin)
This is the DRSC's basic protocol for fixation with formaldehyde and staining with DAPI and/or phalloidin. It is also appropriate for fixation prior to immunostaining, although some antibodies, cell types, target proteins, etc. might require a modified protocol.
Add the fluorescent phalloidin staining solution to each coverslip, then incubate for 30–60 minutes at room temperature. Place the coverslips in a covered container to prevent evaporation during the incubation.
Phalloidin Staining Protocol - Washington University in St. Louis
1997年7月11日 · Phalloidin Staining Protocol. Rhodamine-Phalloidin Staining. Last updated 7/11/97 JAC. 1. Rinse cells or tissue in PBS briefly to remove media components. 2. Fix cells or tissue in 1-2% paraformaldehyde in PBS (freshly prepared) for 15 min at RT.
Phalloidin labeling protocol for optimal F-actin staining
Fluorescent phalloidin conjugates can be used to stain actin filaments (F-actin) in fixed specimens, making it an essential tool to visualize the actin skeleton of cultured cells, tissue, whole organs, or plants.
Fluorescent Phalloidin: A Practical Stain for Visualizing Actin ...
2018年7月1日 · Since its initial use to stain actin in the late 1970s, phalloidin and its fluorescent derivatives remain the gold standard for visualizing cellular actin filaments. Compared to IHC staining, phalloidin derivatives offer significant advantages for labeling actin.
Phalloidin Staining for F-Actin in Hepatic Stellate Cells
2023年5月30日 · Therefore, stains with actin-specific antibodies and phalloidin conjugates for actin staining are widely used to visualize actin structures in myofibroblasts. Here we present an optimized protocol for F-actin staining for hepatic stellate cells using a fluorescent phalloidin.
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