Peptide-guided lipid nanoparticles deliver mRNA to the neural
2023年1月11日 · Lipid nanoparticles (LNPs) are the most clinically advanced nonviral platform for mRNA delivery. Their worldwide dissemination as part of the coronavirus disease 2019 vaccine has proven their safety and efficacy (1). Our efforts have focused on using LNP mRNA delivery for the treatment of inherited retinal degenerations (IRDs).
Peptide-Lipid Interactions: Experiments and Applications
2013年9月12日 · The interactions between peptides and lipids are of fundamental importance in the functioning of numerous membrane-mediated cellular processes including antimicrobial peptide action, hormone-receptor interactions, drug bioavailability across the blood-brain barrier and viral fusion processes.
Lipid-peptide nanocomplexes for mRNA delivery - ScienceDirect
2022年8月1日 · Here, we describe the systematic optimisation of lipid-peptide nanocomplexes for the delivery of mRNA in two murine cancer cell types, B16-F10 melanoma and CT26 colon carcinoma as well as NCI-H358 human lung bronchoalveolar cells.
Direct protein-lipid interactions shape the conformational
2018年10月8日 · A major finding of this study is the identification of direct lipid–protein interactions between the charge-relay networks of MFS transporters and the amine headgroup of the PE phospholipid.
The systematic analysis of protein–lipid interactions comes of age
2015年10月28日 · We believe that a global atlas of protein–lipid interactions will benefit biology and medicine by shedding light on the role of lipids with 'orphan' bioactive activity; by deciphering novel...
Peptide meets membrane: Investigating peptide-lipid …
2023年8月1日 · Peptide–lipid interactions play an important role in defining the mode of action of drugs and the molecular mechanism associated with many diseases. Model membranes consisting of simple lipid mixtures mimicking real cell membranes can provide insight into the structural and dynamic aspects associated with these interactions.
Mechanism of Membrane Fusion: Interplay of Lipid and Peptide
2022年4月18日 · Lipid composition can modulate membrane fusion by altering membrane physical properties, and the structure, organization, and dynamics of fusion proteins and peptides (Meher and Chakraborty 2019). Further, some lipids directly bind to fusion proteins and thereby controlling their fusogenic behavior (Moon and Jun 2020 ).
Peptide-Folding Triggered Phase Separation and Lipid Membrane ...
Peptide-Folding Triggered Phase Separation and Lipid Membrane Destabilization in Cholesterol-Rich Lipid Vesicles. Johanna Utterström. , Hanna M. G. Barriga. , Margaret N. Holme* , Robert Selegård. , Molly M. Stevens* , and. Daniel Aili* Cite this: Bioconjugate Chem. 2022, 33, 4, 736–746. Publication Date: April 1, 2022.
Capture of endogenous lipids in peptidiscs and effect on protein ...
2024年4月19日 · Peptidisc also enables re-incorporating specific lipids to fine-tune the protein microenvironment and assess the impact on downstream protein associations. This study offers a first look at the illusive protein-lipid interaction specificity, laying the path for a systematic evaluation of lipid identity and contributions to membrane protein ...
Binding-induced lipid domains: Peptide-membrane interactions …
2024年2月8日 · The combination of 2D IR and fluorescence anisotropy enables us to study subtle differences in the localized region of lipid membranes upon peptide binding. The results show that negatively charged lipids enhance the binding of MARCKS-ED to lipid vesicles.