Microscale thermophoresis - Wikipedia
Microscale thermophoresis (MST) is a technology for the biophysical analysis of interactions between biomolecules. Microscale thermophoresis is based on the detection of a temperature-induced change in fluorescence of a target as a function of …
MicroScale Thermophoresis: Interaction analysis and beyond
2014年12月5日 · MicroScale Thermophoresis (MST) measures dissociation constants down to 1 picomolar. MST can dissect different binding mechanism by distinct thermophoresis signals. MST quantifies dissociation constants in 100% cell lysate. MST can be used to determine thermodynamic parameters for interactions.
MicroScale Thermophoresis (MST) | Center for Macromolecular …
MicroScale Thermophoresis (MST) is an immobilization-free technology for measuring biomolecular interactions. The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in the molecular hydration shell, charge, or size.
实验笔记丨分子间相互作用分析——微量热泳动（MST）技术介绍
MST技术将荧光检测的精确性和热泳动的差异性及灵敏性结合起来，提供了一种灵活、稳定和快速检测分子间相互作用的方法，在分析分子相互作用中有独特的优势，不依赖于分子大小、种类的影响，甚至水化层的微小变化也可检测到；可检测溶液中低至pM浓度的分子间相互作用；样品消耗量在μL级；可以在复杂缓冲液中包括血浆和细胞裂解液中进行结合分析；还可以分析结合模式和 …
Microscale Thermophoresis (MST) - SpringerLink
2018年6月30日 · Microscale thermophoresis (MST) is a biophysical technique that measures the strength of the interaction between two molecules by detecting variations in fluorescence signal as a result of an IR laser-induced temperature change.
2023年5月3日 · MicroScale Thermophoresis (MST) is an immobilization-free technology for measuring biomolecular interactions. The MST instrument detects the motion of fluorescent molecules along a microscopic temperature gradient, which reflects changes in the molecular hydration shell, charge, or size.
MicroScale Thermophoresis: A Rapid and Precise Method to …
2017年9月13日 · Microscale Thermophoresis (MST) is a rapid and precise method allowing for quantitative analysis of molecular interactions in solution on a microliter scale. The technique is based on the movement of molecules in temperature gradients, which is referred to as thermophoresis, and depends on molecule size, charge, and hydration shell.
Protein-binding assays in biological liquids using microscale ...
2010年10月19日 · In this study, we present a sample-efficient, free-solution method, termed microscale thermophoresis, that is capable of analysing interactions of proteins or small molecules in biological...
PD-1/PD-L1 binding studies using microscale thermophoresis
2017年12月15日 · Microscale thermophoresis (MST) is a powerful new method for the quantitative analysis of protein-protein interactions (PPIs) with low sample consumption. In addition, one of...
Microscale thermophoresis as a powerful growing analytical …
2022年7月28日 · It gives a summarized overview about both the state-of the art and the development in the field of microscale thermophoresis. The principle of microscale thermophoresis is also described supported with self-created illustrations.