A role for APX1 gene in lead tolerance in Arabidopsis thaliana
2017年3月1日 · In this study, enhanced Pb tolerance in apx1-3 mutant was associated with GSH-dependent PC synthesis, however, KO-APX1 mutants showed significantly decreased Pb content. Therefore, from these results, we can conclude that efflux pumping is a major mechanism contributing to Pb tolerance of KO-APX1 mutant and the GSH-dependent PC synthesis ...
S-Nitrosylation Positively Regulates Ascorbate Peroxidase Activity ...
2015年2月9日 · We found that S -nitrosylation of APX1 at cysteine (Cys)-32 enhances its enzymatic activity of scavenging hydrogen peroxide, leading to the increased resistance to oxidative stress, whereas a substitution mutation at Cys-32 causes the reduction of ascorbate peroxidase activity and abolishes its responsiveness to the NO -enhanced enzymatic activity.
Loss‐of‐function mutations in the APX1 gene result in enhanced …
2016年5月5日 · The glutathione redox state (GSH/GSSG) in the plants grown normally was 9.94 for the WT and 9.87, 9.66 and 9.89 (a relatively normal ratio) for the mutant apx1-3, apx1-4 and apx1-5, respectively, while those plants that were treated with Se showed a ratio of 9.47 for the WT and 13.22, 13.65 and 13.43 for the mutant apx1-3, apx1-4 and apx1-5 ...
Rapid Accumulation of Glutathione During Light Stress in …
2018年5月25日 · These results suggest that apx1 plants do not accumulate significant levels of free glutathione during light stress. Because we observed a link between NO and GSH accumulation in wild-type plants , we also measured the accumulation of …
Novel insight into functions of ascorbate peroxidase in higher …
2023年8月1日 · The dual catalytic activity of OgAPX1 confers greater protection against salt and heat tolerances when overexpressed in Arabidopsis compared with overexpression of AtAPX1. Aside from OgAPX1, recombinant cytAPXs from rice, maize and soybean also possess GSH oxidation activity that refers to glutathione peroxidase (GPX) [60].
Regulation of Dual Activity of Ascorbate Peroxidase 1 From
2021年6月13日 · APX1 is a cytosolic ascorbate peroxidase that scavenges reactive oxygen species and acts as a molecular chaperone. The protein structure and function of APX1 are regulated by posttranslational modifications and stress conditions.
Loss-of-function mutations in the APX1 gene result in enhanced
Moreover, enhanced Se resistance of the mutants was associated with glutathione (GSH), which had the higher expression level of GSH1 gene involved in GSH synthesis and consequently increased GSH content. Our results provide genetic evidence indicating that loss-of-function of APX1 results in tolerance to Se stress.
PtomtAPX , a mitochondrial ascorbate peroxidase, plays an
2019年12月20日 · Our findings demonstrates a mitochondrial location for one APX isoform, and provide valuable insight into the mechanism which ROS balance is modulated by AsA-GSH cycle in mitochondria.
Genome-wide identification of ascorbate-glutathione cycle …
2021年9月30日 · The article reports the genome-wide identification and evolution of AsA-GSH cycle genes in soybean and other leguminous species. It also analyzes the gene expression patterns of AsA-GSH cycle genes in soybean tissues and under different stress conditions.
Our results provide genetic evidence indicating that loss-of-function of APX1 results in tolerance to Se stress. Key-words: mutant apx1-3; APX1 gene; selenite; glutathione; glutathione peroxidase. INTRODUCTION.