Improved Culture Conditions for the Growth and Detection of Borrelia …
In this report we present a method to cultivate Borrelia spirochetes from human serum samples with high efficiency. This method incorporates improved sample collection, optimization of culture media and use of matrix protein.
Comparison of Growth of Borrelia afzelii, Borrelia garinii, and ...
Lyme borreliosis is caused by the spirochete Borrelia burgdorferi sensu lato, a fastidious bacterium that replicates slowly and requires special conditions to grow in the laboratory. Borrelia isolation from clinical material is a golden standard for …
Microbiology of Borrelia burgdorferi - PubMed
This article reviews the natural history, taxonomy, physical structure, growth requirements, and molecular structure of Borrelia burgdorferi sensu lato, the causative agent of Lyme disease. These spirochetal bacteria are maintained in nature through an …
Comparison of Growth of Borrelia afzelii, Borrelia garinii, and
2016年6月16日 · Lyme borreliosis is caused by the spirochete Borrelia burgdorferi sensu lato, a fastidious bacterium that replicates slowly and requires special conditions to grow in the laboratory. Borrelia isolation from clinical material is a golden standard for microbiological diagnosis of borrelial infection.
(A) Growth curve of B. burgdorferi strain B31 in vitro. (B)...
Lyme disease, which is caused by the spirochete Borrelia burgdorferi is the most prevalent tick-borne illness in the world today and has grown into a major public health problem during the last...
Growth parameters of Borrelia burgdorferi sensu stricto at …
1998年12月1日 · Growth of Borrelia burgdorferi sensu stricto (prototype strain B-31) was studied in Barbour-Stoenner-Kelly BSK-H liquid medium, supplemented with 4.5 % rabbit serum and antibiotics (phosphomycin, rifampicin), at various temperatures to early stationary growth phase. The number of cells was determined by darkfield microscopy.
Evaluation of a New Culture Medium for Borrelia burgdorferi
We evaluated the new MPM medium for the growth of Borrelia burgdorferi. All 18 blood samples from 17 patients with Lyme disease were negative. Growth studies showed that by day 4, most organisms in MPM were not viable. Our results reinforce the use of BSK medium as the primary choice for growing B. burgdorferi.
RNA-Seq of Borrelia burgdorferi in Multiple Phases of Growth
2016年10月5日 · To address that concern, we performed RNA sequencing (RNA-Seq) analysis of B. burgdorferi cultures at early-exponential, mid-exponential, and early-stationary phases of growth. We found that expression of nearly 18% of annotated B. burgdorferi genes changed significantly during culture maturation.
Culture of Borrelia burgdorferi on six solid media - PubMed
Six solid substrates were compared for their support of growth of ten isolates of Borrelia burgdorferi, the causative agent of Lyme borreliosis. The following substrates with or without rabbit serum and bovine serum albumin were tested: BSK agar, BHIAM agar, TAROM agar, MEM agar, MKP agar and PMR ag …
Culture of Borrelia burgdorferi - PMC - PubMed Central (PMC)
None of the patient samples showed growth of B. burgdorferi. The control culture in BSK-H grew luxuriantly, as evidenced by the appearance of spirochetes in the AO stain and a positive PCR result. The control organism failed to grow in MPM medium.