Alanine scanning - Wikipedia
In molecular biology, alanine scanning is a site-directed mutagenesis technique used to determine the contribution of a specific residue to the stability or function of a given protein. [1]
Analysis of Large-Scale Mutagenesis Data To Assess the Impact of …
2017年7月26日 · The first application of scanning mutagenesis used alanine substitutions to identify positions in human growth hormone important for receptor binding (Cunningham and Wells 1989). Alanine was chosen because it represents a deletion of the side chain at the β …
Exploring amino acid functions in a deep mutational landscape
Similarly, on a gene‐by‐gene level, alanine scanning experiments have shown the power of mutations to infer positional functions in proteins using mutations, for example mapping hGH receptor interactions (Cunningham & Wells, 1989) and the CD4‐binding site (Ashkenazi et …
Alanine Scanning Mutagenesis: A Versatile Approach to Map …
2021年4月21日 · During the ongoing COVID-19 pandemic, investigators have harnessed the versatility of alanine scanning mutagenesis to map "hot spots" in SARS-CoV-2. For example, most recently, Suryadevara et al. 2021 leveraged alanine scanning to map new neutralizing epitopes in the Spike protein.
Whole-protein Alanine-scanning Mutagenesis of Allostery: a Large ...
Mutations that reduce alanine binding are localized near the A-C domain interface, which is the location of the amino acid binding site. However, there are also mutations on the N-terminus domain that influence alanine binding.
Alanine Scanning Mutagenesis: A Versatile Approach to Map
2021年4月20日 · Learn about how alanine scanning mutagenesis helps to map amino acid residues essential for protein functions and protein-protein interactions facilitating immunogen and antibody design and supporting therapeutic development.
BAlaS: fast, interactive and accessible computational alanine …
2020年1月13日 · Alanine-scanning mutagenesis is an experimental technique where residues in a protein of interest are sequentially mutated to alanine (Cunningham and Wells, 1989). The aim is to identify those residues that contribute the most to the free energy of interaction.
Alanine-stretch scanning mutagenesis: a simple and efficient …
1997年1月1日 · Our alanine stretch mutagenesis method can be used as a probe to rapidly scan a whole protein sequence in search of secondary structures or to characterize the catalytic or functional role of a stretch of residues.
High-Throughput Alanine Scanning
2012年6月15日 · Alanine (Ala) scanning is a widely used mutagenesis approach in which residues in a target protein are systematically substituted for alanine at selected positions by site-directed mutagenesis,...
Using large-scale mutagenesis to guide single amino acid …
2017年5月22日 · Alanine scanning mutagenesis is a widely-used method for identifying protein positions that are important for function or ligand binding. Alanine was chosen because it is physicochemically innocuous and constitutes a deletion of the side chain at the β- carbon.