Intro to Microtomy: Procedure for Preparing & Sectioning Tissue
"Microtomy and Paraffin Section Preparation" is a great training aid for new microtomists and an excellent refresher for experienced operators. All the essential aspects of cutting paraffin sections are covered, including: Safety; Microtome setup; Microtome blades; Trimming, facing and roughing blocks; Techniques for consistent paraffin sections
Microtomy: Cutting Formalin-Fixed, Paraffin-Embedded …
2018年12月12日 · Here we describe the process of cutting paraffin embedded sections using a rotary microtome. We also highlight the possible pitfalls that may arise and discuss how to avoid them. Proper microtomy technique is essential in preparing formalin-fixed paraffin embedded tissue for biobanking purposes.
a training aid for newcomers to microtomy and paraffin section preparation and as a refresher course for more experienced histologists. It covers the essential elements for set‑up and safe operation of a rotary microtome to prepare paraffin sections. Some of the more common faults seen in sections are illustrated and troubleshooting
石蜡切片技术 (Paraffin Section) | Protocols | MCE
Next, use a microtome to cut the paraffin-embedded tissue into thin sections for observation under a microscope. This technology can preserve the tissue structure, making it suitable for histological sectioning and immunohistochemistry experiments, providing reliable histological information for disease diagnosis and biomedical research.
Microtomy: Cutting Formalin-Fixed, Paraffin-Embedded Sections
Therefore, proper microtomy technique is essential in the preparation of formalin-fixed, paraffin-embedded tissue for biobanking purposes. Here we describe the process of cutting paraffin embedded sections using a rotary microtome. We also highlight the possible pitfalls that may arise and discuss how to avoid them.
Protocol for preparing formalin-fixed paraffin-embedded …
2024年6月21日 · Here, we present a protocol for using spatial transcriptomics in bone and multi-tissue musculoskeletal formalin-fixed paraffin-embedded (FFPE) samples from mice. We describe steps for tissue harvesting, sample preparation, paraffin embedding, and FFPE sample selection.
Microtomes and Microtomy Products - Leica Biosystems
Creating great paraffin sections using a rotary microtome takes a great deal of skill and experience. "Microtomy and Paraffin Section Preparation" is a great training aid for new microtomists and an excellent refresher for experienced operators. Learn More.
Tissue Microtomy: Principle and Procedure | SpringerLink
2018年6月9日 · It is the main instrument by which we cut the embedded tissue in the paraffin block as thin section. The different types of microtomes in the traditional histology laboratory are: Rotary microtome. Rocking microtome. Base sledge microtome. Sliding microtome. Cryomicrotome. Ultramicrotome. Laser microtome (a)
Preparation and Sectioning of Paraffin-Embedded Tissue for
2024年11月6日 · Paraffin-infiltrated tissues can be solidified to prepare paraffin blocks for sectioning with a microtome. Using this method, we can section rice shoot apex and immature inflorescence. Produced sections can be treated with tissue-staining reagents, such as toluidine blue O ( see Fig. 1 ), or used for in situ hybridization analysis if the ...
To produce paraffin section slides, trim and section blocks on a rotary microtome following the procedure below: 1) Pre-warm a floating water bath containing fresh dH2O. The typical water bath temperature is 37-42°C. However, depending on the paraffin type, the floating temperature may need to be adjusted to 34-45°C.