Origin and use of crystallization phase diagrams - PMC
Framework and components of a crystallization phase diagram. The ‘crystallization phase diagram’ is a useful operational framework for visualizing processes during crystallization. It is …
In this article I describe methods for measuring the phase diagram of an aqueous solution of a globular pro-tein, focusing on the crystalline (Section 2) and liquid phases (Section 3) which …
Crystallization by Phase Diagram - Douglas
Each set of 24 wells is represented by a horizontal line on the figure below. To illustrate the type of crystallization results obtained, we will show a typical line of 24 trials. The protein …
Solutes don’t crystallize! Insights from phase diagrams demystify …
2024年10月2日 · Thermodynamics requires that the nutrient (solvent) be the crystallizing phase. This perspective provides tools to rigorously characterize a phase diagram to explicitly …
Crystallization phase diagrams are frequently used to conceptualize the phase relations and also the processes taking place during the crystallization of macromolecules.
Protein crystallization and phase diagrams - ScienceDirect
2004年11月1日 · In this article, methods to determine the phase diagram of an aqueous solution of a globular protein are described, focusing on the solid (crystal) and condensed liquid states. …
A phase diagram provides information about crystal nucle-ation and growth conditions as a function of protein concentra-tion and thermodynamic parameters such as temperature, pH, …
The process of crystallization can be differentiated into two steps: (1) the nucleation process, and (2) crystal growth. With correctly selected conditions, crystal nucleation and growth can occur …
Understanding protein crystallization on the basis of the phase diagram ...
1999年1月15日 · First we discuss the protein–water phase diagram, which is the basis for all phase separation processes, and we try to specify optimal conditions for crystal growth. Next …
Protein crystallization and phase diagrams - PubMed
In this article, methods to determine the phase diagram of an aqueous solution of a globular protein are described, focusing on the solid (crystal) and condensed liquid states. The use of …