Instant polarized light microscopy for imaging collagen ...
Collagen fibers are a primary load-bearing component of connective tissues and are therefore central to tissue biomechanics and pathophysiology. Understanding collagen architecture and behavior under dynamic loading requires a quantitative imaging technique with simultaneously high spatial and temporal resolutions.
High-resolution confocal and light-sheet imaging of collagen 3D …
2023年4月21日 · Here, we introduce a simple and robust method of whole-mount collagen staining with the ‘Fast Green’ dye that provides unmatched visualization of collagen 3D network architecture, via confocal or light-sheet microscopy, compatible with solvent-based tissue clearing and immunostaining.
Collagen: quantification, biomechanics and role of minor ... - Nature
2020年7月20日 · In this Review, we introduce the roles of collagen subtypes and crosslinks and describe modern assays that enable a deep understanding of tissue physiology and disease states.
Structural characteristics of the collagen network in human …
2011年12月1日 · Characterizing normal collagen structure establishes a benchmark against which repair cartilage can be compared, and analyses of degraded cartilage identify structural features that would ideally be avoided.
Instant polarized light microscopy for imaging collagen ...
We demonstrate that IPOL allows quantitative characterization of micron-scale collagen fiber architecture at full camera frame rates (156 frames/second herein). Keywords: biomechanics; collagen; deformation; polarized light microscopy.
Structured polarized light microscopy for collagen fiber structure …
We introduce structured polarized light microscopy (SPLM), an imaging technique that combines structured light illumination with PLM to allow imaging and measurement of collagen fiber properties in thick ocular tissues.
Colorful Protein-Based Fluorescent Probes for Collagen Imaging
2014年12月9日 · To allow for the use of CNA35-based probes in a broader range of applications, we here present a toolbox of six genetically-encoded collagen probes which are fusions of CNA35 to fluorescent proteins that span the visible spectrum: mTurquoise2, EGFP, mAmetrine, LSSmOrange, tdTomato and mCherry.
Real-time polarization microscopy of fibrillar collagen in ...
2021年9月24日 · In this paper, we evaluate previously described polychromatic polarization microscope (PPM) to visualize collagen fibers with an optically generated color representation of fiber orientation and...
Model-Based Approach for the Semi-Automatic Analysis of Collagen …
2023年2月24日 · We propose an efficient semi-automatic computational approach for the quantification of collagen content from the analysis of PLM images of birefringent histological sections. The method is based on a physical model of light-sample interaction and birefringence effect production.
MR Imaging and Spectroscopy of Collagen | SpringerLink
2024年2月20日 · Experiments with aqueous collagen solutions and previous works investigating collagen signals or signals from bound water to collagen in various organ systems will be reviewed. Different magnetic resonance (MR) investigation methods are compared, and a new method with high spatial and spectral resolution is presented.