Pregna-D′-pentarane structure influences progesterone receptor …
2002年4月1日 · Electrophoretic mobility shift assay was used to determine whether pregna-D′-pentaranes allow progesterone receptor (PR) from rat uterine cytosol to bind hormone response element (HRE)-containing oligonucleotide duplexes and to measure the affinity of this interaction.
Progesterone receptor transcription and non-transcription …
2003年11月1日 · DNA bound receptors increase or decrease rates of gene transcription by influencing recruitment of RNA polymerase II to the initiation site. Through protein–protein interaction, hormone activated PR recruits coactivators that serve as essential intermediates for transmitting signals from the receptor to the transcription initiation complex.
Fluorescence Anisotropy Microplate Assay for Analysis of Steroid ...
2024年2月9日 · The steroid receptors including estrogen, progesterone (ER and PR, respectively), and androgen receptors comprise a subfamily of the larger nuclear receptor superfamily of transcription regulators. The receptors contain discrete functional domains for hormone binding, transactivation, and DNA binding [DNA binding domain (DBD)].
Progesterone receptor and the mechanism of action of progesterone …
1995年6月1日 · Currently available progesterone antagonists have been suggested to fall into two categories based on differences in how they interact with and inactivate the progesterone receptor (PR). The anti-progestin ZK98299 (Type I) impairs PR association with DNA, while Type II compounds (RU486, ZK112993, ZK98734) promote PR binding to DNA.
Structure of the Progesterone Receptor-Deoxyribonucleic Acid …
We have determined the structure of the progesterone receptor (PR)-DBD-CTE DNA complex at a resolution of 2.5 Å, which revealed binding of the CTE to the minor groove flanking the HREs. Alanine substitutions of the interacting CTE residues reduced affinity for inverted repeat HREs separated by three nucleotides, and essentially abrogated ...
Antagonists RU486 and ZK98299 Stimulate Progesterone Receptor …
1998年4月1日 · The major findings of this study are that previously designated type I (ZK98299) and type II (RU486, ZK98734, and ZK112993) progesterone antagonists enhance sequence-specific PR-DNA binding activity in whole cells and in vitro by a modified EMSA procedure.
Progesterone Inhibits Basal and Gonadotropin-Releasing …
In this study, we investigated the mechanism of progesterone repression of LHβ gene expression using immortalized gonadotrope-derived LβT2 cells. The progesterone suppression of both basal and GnRH-induced LHβ gene expression occurs in a hormone- and receptor-dependent manner.
Mechanism of high-mobility group protein B enhancement of progesterone …
2008年5月12日 · The DNA-binding domain (DBD) of progesterone receptor (PR) is bipartite containing a zinc module core that interacts with progesterone response elements (PRE), and a short flexible carboxyl terminal extension (CTE) that interacts with …
A molecular toolbox to study progesterone receptor signaling
2023年11月29日 · Progesterone receptor (PR) signaling is required for mammary gland development and homeostasis. A major bottleneck in studying PR signaling is the lack of sensitive assays to measure and visualize PR pathway activity both quantitatively and spatially. Here, we develop new tools to study PR signaling in human breast epithelial cells.
Progesterone Receptors, their Isoforms and Progesterone …
This review discusses mechanisms by which progesterone receptors (PR) regulate transcription. We examine available data in different species and tissues regarding: 1) regulation of PR levels; and 2) expression profiling of progestin-regulated genes ...