The mechanism of amyloid fibril growth from Φ -value analysis
4 天之前 · This model describes amyloid elongation in a manner consistent with findings from multiple studies in the linear growth regime 12,15,16,17 and furthermore allows us to accurately define the ...
The Amyloid-β Pathway in Alzheimer’s Disease - Nature
2021年8月30日 · The Aβ is a 4 kDa fragment of the amyloid precursor protein (APP), a larger precursor molecule widely produced by brain neurons, vascular and blood cells (including platelets), and, to a lesser...
Amyloid-β oligomers in cellular models of Alzheimer’s disease
In this review, we discuss physicochemical properties of AβOs and their impact on different brain cell types in AD. Additionally, we summarize three decades of studies with AβOs, providing a compelling bulk of evidence regarding cell-specific mechanisms of toxicity.
Amyloid formation as a protein phase transition - Nature
2023年6月27日 · We summarize progress in the efforts to describe the essential biophysical features of amyloid self-assembly as a nucleation-and-growth process and discuss how master equation approaches can...
Amyloid Precursor Protein and Alzheimer’s Disease - PMC
Amyloid precursor protein (APP) is a membrane protein considered to play a main role in AD pathology. It is known that APP in physiological conditions follows a non-amyloidogenic pathway; however, it can proceed to an amyloidogenic scenario, which leads to the generation of extracellular deleterious Aβ plaques.
Biology of Amyloid: Structure, Function, and Regulation
2010年10月13日 · The infectivity of an amyloid depends on the concentration of soluble and aggregated amyloid protein, the growth rate of the amyloid, and the rate of breakage of the amyloid fiber into seeds. According to this theoretical framework, manipulation of these variables could transform any amyloid into a prion ( Riek, 2006 ).
A cellular model of amyloid precursor protein processing and
2014年2月15日 · Results: In BE (2)-M17 cells, both RNA (i.e., RT-PCR, RNA sequencing) and protein analyses (i.e., Western blots, ELISA), show endogenous expression of critical components of the amyloidogenic pathway, APP-cleavage intermediates CTF83 and CTF99, and final cleavage products Aβ40 and Aβ42.
Lysozyme: A model protein for amyloid research - ScienceDirect
2011年1月1日 · Owing to its easy availability, small size, and excellent solubility in aqueous medium, HEWL can therefore serve as a model protein to investigate protein aggregation in the context of developing a detailed understanding of lysozyme amyloidosis. A variety of conditions have been developed over the years to induce aggregation in this protein.
Amyloid beta: structure, biology and structure-based therapeutic ...
Amyloid beta peptide (Aβ) is produced through the proteolytic processing of a transmembrane protein, amyloid precursor protein (APP), by β- and γ-secretases. Aβ accumulation in the brain is proposed to be an early toxic event in the pathogenesis of Alzheimer's disease, which is the most common form of dementia associated with plaques and ...
The Amyloid Precursor Protein Has a Flexible Transmembrane
2012年6月1日 · Amyloidogenic cleavage of full-length amyloid precursor protein (APP) by β-secretase generates the transmembrane protein C99 (APP 672-770, also known as β-CTF, fig. S1). The transmembrane domain (TMD) of C99 is then processively but imprecisely cleaved by γ-secretase to release the Aβ polypeptides ( 6 , 7 ).