In the last decade, two techniques have revolutionized fluorescence microscopy: the development of genetically encoded fluorophores, such as GFP 5,6 and the invention of two-photon microscopy 7.

A miniature head-mounted two-photon microscope small enough for a rat to carry allows researchers to visualize neuronal signaling while the animal freely interacts with its environment. It is ...

In Vivo Two-Photon Based Imaging of Cortical Blood Flow Two-photon imaging of cortical blood flow through an acute cranial window. Blood vessels were visualized via injection of a fluorescein molecule ...

Healthy blood ... using two high-resolution 3D mapping techniques: serial two-photon tomography—a technique that creates a series of stacked 2D images—and light sheet fluorescence microscopy ...