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普洛麦格中文通讯 第八期 2003
Oligo 1(top strand for sense):5 ... 2-18 G 19 (sense)-AAGTTCTCT(loop)-C 19 N 18-2 G 1 (antisense)-AA-5' 图3.产生siRNA 或发夹siRNA的模板所需要的DNA寡聚核苷酸。靶mRNA的正义序列被认为是蛋白编码序列。 图4:比较用化学合成的或用T7 RiboMAXTM Express RNAi ...
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